Aldon® AP Biology Investigation #8: Biotechnology: Bacterial Transformation

With this kit, students will genetically engineer bacteria through transformation with a plasmid that confers antibiotic resistance. Then, they will examine the number of resistant bacteria to determine the efficiency of the transformation procedure. Kit includes: enough materials for 8 lab groups: 30 ml Sterile Calcium Chloride 0.05M, 8 Pipet Bulbs, 16 Microcetrifuge Tubes 1.5 ml, 40 Petri dishes, 1 Luria Broth 9ml Tube, 16 sterile volumetric Pipet, 8 Micropipet with plunger 10ul, 8 sterile Inoculating loops, 4 Luria Agar 200ml, a teacher’s manual, and a student study guide (copymasters are included). A coupon is also included to be redeemed for the following perishable materials: 2 bottles of 0.02g Ampicillin, 1 Tryptic Soy Broth Tube 9ml, 1 sterile Pipet (Disposable), 1000 ng pUC 19 Plasmid, 1 Tryptic Soy Agar Slant 6ml, and 1 E. Coli Culture (freeze dried). Kit meets AP Science Practices 1,3,5,6 and 7, and Big Idea 3.